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C. Robertson McClung C.Robertson.McClung at Dartmouth.EDU
Tue May 17 09:57:10 EST 1994

How are you making your probes?  If you are cutting out bands derived from
plasmid vetors, your "gel-purified" inserts are almost certainly contaminated
with plasmid vector which might hybridize to the vector as you describe.

Perhaps you should amplify your probe DNA by PCR?  Or purify DNA from an
alternate vector (a phage?) that has no homology with the library vector?

Good luck.

rob mcclung
Dartmouth College

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