Some months ago on the net, there was some discussion of how to
remove polysaccharide contaminants from protein preps prior to
running SDS-PAGE gels. I did not save this correspondance, and now
find that I need it!
We are isolating a soluble protein using immunoaffinity
chromatography, and find that our crude protein preps contain much
polysaccharide that gradually precipitates. This is wreaking havoc
on our column. Any suggestions for how to remove polysaccharides
from the protein solution?
Please post replies to:
kate at bio.tamu.edu