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polysaccharide contaminants in protein preps

Wed May 4 11:36:02 EST 1994

Some months ago on the net, there was some discussion of how to 
remove polysaccharide contaminants from protein preps prior to 
running SDS-PAGE gels.  I did not save this correspondance, and now 
find that I need it!

We are isolating a soluble protein using immunoaffinity 
chromatography, and find that our crude protein preps contain much 
polysaccharide that gradually precipitates.  This is wreaking havoc 
on our column.  Any suggestions for how to remove polysaccharides 
from the protein solution?

Please post replies to:

Kate VandenBosch
kate at bio.tamu.edu

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