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two-hybrid system

ZHOUJM at PURCCVM.BITNET ZHOUJM at PURCCVM.BITNET
Fri Jun 17 14:01:53 EST 1994


I am trying to use two-hybrid system to identify proteins that
interact with a tomoto protein.  Does anyone out there has had
experience as to construct a cDNA library for this purpose? What
is the best system to use?  Any successful example of cloning a
plant gene using this strategy?

Jianmin Zhou



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