>>I am trying to use two-hybrid system to identify proteins that
>interact with a tomoto protein. Does anyone out there has had
>experience as to construct a cDNA library for this purpose? What
>is the best system to use? Any successful example of cloning a
>plant gene using this strategy?
>You might try connecting to the AAtDB gopher and looking in our Dept of
Mol. Biology directry for the detailed information from Roger Brent's lab
on the Protein - Protein Interaction trap. the URL is