Dear arabidopsis netters
1- I'm looking for a protocol to extract a cimeric protein containing a
7-transmembrane-protein fused to GUS w/o affecting GUS activity.
2- Does anyone have a working protocol to detect the activity of a
GUS-fusion protein on SDS-PAGE or a membrane?
3- Does anyone have a "good" antibody to GUS that can detect a
GUS-fusio protein on western as well as for immunolocalization?
4- Does anyone know if GUS is active as a monomer?
I will appreciate any comments. Thanks Maor.
Plant Res. Lab.
E-mail: 22693MGR at MSU.bitnet