Gijs van Rooijen wrote:
> It has been well documented that GUS tolerates protein fusions to its N-terminal
> end. Has anybody out there any experience (or knows of any references), in which
> fusions were made to the C-terminal end of GUS. I would like to know whether
> these fusions still show GUS activity. If not, does anybody know why?
> Thanks in advance,
One good example of high activity GUS C-terminal fusions is the
work in Jim Carrington's laboratory where potyviral proteins and
their deletion derivatives were fused to GUS to identify nuclear
transport signals (Plant Cell 3: 953-962).
Brian H. Taylor *
Dept. of Biology, Texas A&M University * When we try to pick out
College Station, TX 77843-3258 * anything by itself, we
* find it hitches to every-
Phone: (409) 845-7754 * thing in the universe.
FAX: (409) 845-2891 * John Muir