Three postdoctoral positions are available in the Biochemistry
and Physiology Department, Rothamsted Experimental Station, from
1st October (or as soon as possible thereafter). The projects are
part of phase II of the AFRC's coordinated programme in Plant
1. Molecular studies of plant secondary metabolism (ref. 956).
A three year post to isolate and characterise the gene for
an enzyme involved in plant monoterpene biosynthesis. A
yeast expression library will be screened by a novel
approach in which a histochemical assay is used to assay
for enzyme activity in the transformed yeast cells. The
work will involve cDNA library construction, screening,
sequence analysis and expression studies using northern
blotting and in situ hybridization. (David Hallahan, 0582
763133 ext 2264; or Brian Forde, 0582 763133 ext 2238).
2. Gene regulation in Arabidopsis (ref. 957). A four year post
to characterise the primary molecular response to
environmental nitrate in Arabidopsis roots, using
subtractive cDNA library construction, DNA sequencing and
gene expression studies. In addition, nitrate regulatory
mutants will be sought using a novel screening method
applied to a transgenic Arabidopsis line. (Brian Forde,
0582 763133 ext 2238).
3. Structure and function of the Arabidopsis H+/hexose
transporter (ref. 958). This three year post will use
Xenopus oocytes as a heterologous expression system for the
functional expression of a sugar transporter. The hexose
transporter expressed in oocytes will be characterised by
electrophysiological techniques. Site-directed mutagenesis
of the transporter will then be used to investigate the
kinetics and regulation of transporter activity. (Tony
Miller, 0582 763133 ext 2248)
The appointments will be at the Higher Scientific Officer level,
on the salary scale 12,339 - 17,227 (pay award pending).
Applicants should have a BSc Honours degree (First or Upper
Second Class) and at least two years relevant post-qualifying
experience. Preference will be given to candidates with a PhD and
experience in recombinant DNA techniques. Application form
available with further details, from:
Administration Division, AFRC Institute of Arable Crops Research,
Rothamsted Experimental Station, Harpenden, Herts AL5 2JQ, 0582
763133 ext 2753.
Please quote the relevant reference number. Informal enquiries
to the appropriate grant holder, as indicated above.
(or on email via bforde at UK.AC.ARFC.RESA)