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reply to root cDNA library

Robert F. Whittier 101002.2366 at CompuServe.COM
Tue Apr 28 22:16:15 EST 1992


  We have not made any root cDNA libraries, but . . .

  Collecting Arabidopsis root material need not be a horrendous 
task.  Arabidopsis plants can be grown shaking in sterile liquid 
medium (We heard about this originally from Elliot Meyerowitz, but 
several groups apparently do this).  In our lab we sow 5 
surface-sterilized seeds into a 350 ml capacity flask containing 
75 ml Murashige & Skoog medium with 2% sucrose.  After 21 to 25 days 
shaking at 25 degrees C we replace the medium and then harvest 7 to 
10 days later.  This generally yields about 3.5 g of tissue.  We 
have never specifically measured, but eyeballing it I would guess 
that root tissue exceeds leaf tissue considerably.

Mitsui Plant Biotechnology Research Institute

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