In response to a question from Rob Last (Boyce Thompson Inst) on seed
collection from transgenic A.t. plants:
We have reasonable good success when Kan-r shoots of ca 5 mm are directly
transferred to Magenta boxes containing GM medium as described by Valvekens.
When plants develop fully, we use a Magenta vessel coupler (Sigma Plant Cell
Culture Catalogue C0667) to place another vessel on top. The vessels should
then be placed away from heat sources as lights to prevent condensation.
PLant can reach considerable height and make a large number of flowers.
Please summarize all improvements you get on this subject and put it
on the BB.
With best regards, Peter Sijmons (MOGEN, The Netherlands