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Wed Sep 25 17:22:00 EST 1991

This message is in response to eric richards' question about the number of hits
sustained per genome during conventional EMS mutagenesis.  Several years ago
I made such calculations based on observations of mutation frequencies at
several loci following EMS mutagenesis.  I estimated that a M1 population
of 125,000 plants should carry every possible EMS induced mutation and that
any given M2 plant would be either heterozygous or homozygous for approximately
215 mutations.  If you would like to check the logic behind these calculations
they were published :  Haughn and Somerville 1987.  Herbicide resistance at the
whole plant level.  In: Biotechnology in Agricultural Chemistry,  LeBaron,
Mumma, Honeycutt, Duesing eds, ACS Symposium series #334  pp 98-107.

George Haughn

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