Can anyone out there answer this question?
What fairly major protein of approximate MW 40kDa is found in the soluble
protein fraction after grinding Arabidopsis leaves in a buffer containing
1% v/v Triton X-100? Apart from RUBISCO large and small subunits, this is the
strongest band on an SDS-PAGE gel stained with Coomassie Blue.
There's also another fairly strong band of around 50kDa which we haven't
identified - suggestions for candidates for that would also be welcome.
Robin Walters Robert Hill Institute,
BI1RGW at UK.AC.SHEF.IBM Dept. of Mol. Biol. & Biotech.,
(if you're outside University of Sheffield,
the UK reverse the P.O.Box 594,
elements of the node- Western Bank,
name) Sheffield S10 2UH.