People who are interested in ways to clone genes for which mutations are
available should have a look at 'Direct Molecular Identification of the
Mouse Pink-Eyed Unstable Mutation by Genome Scanning' by Brilliant etal.
in Science 252:566. They were interested in cloning a gene for which
a very unstable allele was available (it turned out to be a tandem repeat).
They used a middle-repetitive probe to 'scan' the genome for regions that
were altered in the unstable mutant background compared with an isogenic
wild-type strain. It worked. It should work for homozygous viable
deficiencies, or possibly even for recessive lethals. I am interested
in hearing any reactions to the technique- please reply to the Bboard.
Boyce Thompson Institute