Doug Skrecky wrote:
>> Authors
> Shirahata S. Kabayama S. Nakano M. Miura T. Kusumoto K. Gotoh M.
> Hayashi H. Otsubo K. Morisawa S. Katakura Y.
> Institution
> Institute of Cellular Regulation Technology, Graduate School of Genetic
> Resources Technology, Kyushu University, Fukuoka, Japan.
> Title
> Electrolyzed-reduced water scavenges active
> oxygen species and protects DNA from oxidative damage.
> Source
> Biochemical & Biophysical Research Communications. 234(1):269-74, 1997 May
> 8.
> Abstract
> Active oxygen species or free radicals are considered to cause extensive
> oxidative damage to biological macromolecules, which brings about a variety
> of diseases as well as aging. The ideal scavenger for active oxygen should be
> 'active hydrogen'. 'Active hydrogen' can be produced in reduced
> water near the cathode during electrolysis of
> water. Reduced water exhibits high pH, low
> dissolved oxygen (DO), extremely high dissolved molecular hydrogen (DH), and
> extremely negative redox potential (RP) values. Strongly
> electrolyzed-reduced water, as well as
> ascorbic acid, (+)-catechin and tannic acid, completely scavenged O.-2
> produced by the hypoxanthine-xanthine oxidase (HX-XOD) system in sodium
> phosphate buffer (pH 7.0). The superoxide dismutase (SOD)-like activity of
> reduced water is stable at 4 degrees C for over a month and
> was not lost even after neutralization, repeated freezing and melting,
> deflation with sonication, vigorous mixing, boiling, repeated filtration, or
> closed autoclaving, but was lost by opened autoclaving or by closed
> autoclaving in the presence of tungsten trioxide which efficiently adsorbs
> active atomic hydrogen. Water bubbled with hydrogen gas
> exhibited low DO, extremely high DH and extremely low RP values, as does
> reduced water, but it has no SOD-like activity. These
> results suggest that the SOD-like activity of reduced water
> is not due to the dissolved molecular hydrogen but due to the dissolved
> atomic hydrogen (active hydrogen). Although SOD accumulated H2O2 when added
> to the HX-XOD system, reduced water decreased the amount of
> H2O2 produced by XOD. Reduced water, as well as catalase and
> ascorbic acid, could directly scavenge H2O2. Reduce water
> suppresses single-strand breakage of DNA b active oxygen species produced by
> the Cu(II)-catalyzed oxidation of ascorbic acid in a dose-dependent manner,
> suggesting that reduced water can scavenge not only O2.- and
> H2O2, but also 1O2 and .OH.
Actually, I already read this abstract some months ago when I was doing
research to determined if there was anything to the "clustered water"
that is being hawked in several newsgroups. I am somewhat skeptical
about the purported stability of the stuff. My slant on this result is
that even if true as stated, any effects would very rapidly dissipate
once the stuff was ingested, and very little benefit would be achieved.
However, I would definitely encourage someone to do some in vivo animal,
and later human experiments on this "reduced" water. It might at the
least be beneficial for the mouth and upper GI tract or for IV use.
--Tom
Tom Matthews
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