>From The Worm Breeder's Gazette, 1998, v. 15, No. 2, p. 57.
AN ATTEMPT TO SLOW AGING IN C. elegans.
9. NO POSITIVE EFFECT OF EPITHALAMIN
Vladimir V. Bakaev (e-mail: Vlad at bakaev.nsk.ru), Anatolii V.
Efremov, Vladimir N. Anisimov* P.O. Box 45, Novosibirsk,
630107, Russia *N.N.Petrov Research Institute of Oncology,
St.Petersburg, 189646, Russia
There are data on the life span prolonging effect of pineal
petide preparation epithalamin in mice, rats and Drosophila
melanogaster [1,2].
The purpose of this study was to investigate the effect of dif-
ferent concentrations of epithalamin in water solutions on ne-
matode life span. In this experiment epithalamin was used in
following dilutions : 1:10(5), 1:10(6), 1:10(7), 1:10(8),
1:10(9) and 1:10(10). Three adult animals (3 - 5 days old) were
kept in microtitre wells containing 0,75 ml of liquid medium
(with E. coli and without epithalamin) during 4 hours, then
they were discarded and newborn larvae were transferred in next
wells (with epitalamin in any concentration) every day (one
worm in one well). This investigation was carried out in tempe-
rature +21o C and in the darkness. The obtained results are
presented in the following table.
Table I
‚€€€€€€€€€€€€€€€€€€€€€€€ˆ€€€€€ˆ€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€ƒ
Concentration of Longevity (days)
†€€€€€€€€€€€€€€ˆ€€€€€€€€€€€€€€€‡
epithalamin n mean+/-S.E. maximal
†€€€€€€€€€€€€€€€€€€€€€€€Š€€€€€Š€€€€€€€€€€€€€€Š€€€€€€€€€€€€€€€‡
Control 12 17,4+/-2,5 29
1:10(5) 12 12,8+/-2,1 27
1:10(6) 12 15,1+/-1,9 28
1:10(7) 12 17,1+/-2,2 28
1:10(8) 12 19,6+/-2,2 29
1:10(9) 12 18,8+/-2,0 31
1:10(10) 12 18,7+/-1,7 25
„€€€€€€€€€€€€€€€€€€€€€€€‰€€€€€‰€€€€€€€€€€€€€€‰€€€€€€€€€€€€€€€…
Table II
‚€€€€€€€€€€€€€€€€€€€€€€€ˆ€€€€€ˆ€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€ƒ
Concentration of Longevity (days)
†€€€€€€€€€€€€€€ˆ€€€€€€€€€€€€€€€‡
epithalamin n mean+/-S.E. maximal
†€€€€€€€€€€€€€€€€€€€€€€€Š€€€€€Š€€€€€€€€€€€€€€Š€€€€€€€€€€€€€€€‡
Control 12 23,5+/-2,3 33
1:10(7,5) 12 16,8+/-2,5 34
1:10(8,0) 12 19,3+/-1,7 33
1:10(8,5) 12 13,7+/-1,4 21
„€€€€€€€€€€€€€€€€€€€€€€€‰€€€€€‰€€€€€€€€€€€€€€‰€€€€€€€€€€€€€€€…
Conclusion: If the water solution of epithalamin was applied to
C. elegans during the whole life span in above described condi-
tions, it was not able to prolong the life span of these ani-
mals.
€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€-
1. Anisimov V.N., Khavinson V.Kh., Morozov V.G. Twenty years of
study on effects of pineal peptide preparation: Epithalamin
in experimental gerontology and oncology.
Ann.N.Y.Acad. Sci., 1994, 719: 483-493.
2. Anisimov V.N., Mylnikov S.V., Oparina T.I., Khavinson V.Kh.
Effect of melatonin and pinela peptide preparation epithala-
min on life span and free radical oxidation in Drosophila
melanogaster. Mech. Ageing Dev., 1997, 97: 81-91.
