I am trying to extract mRNA from soil bacteria via direct lysis and I
already aged decades doing it.
I need a very potent RNase-inhibiting agent. I used GITC
(guanidin-isothiocyanate) and DEPC (diethyl-pyrocarbonate) in different
protocols with not really satisfactory results, whereas they really work
fine with pure cultures.
I would be grateful for any advice.
Thanx
Ralf
(Please reply by mail, too, 'cos' I am not following this group
regularly. Thanx again)
--
### We, the Willing,
### led by the Ignorant,
### do the Impossible for the Ungrateful
