selby at lenti.med.umn.edu (Scott Selby (Med-Hem)) wrote:
>I'm looking for a source of mdm-2 cDNA. I'd like to probe some Northern
>blots, and haven't found a source yet. Any help would be appreciated.
>>______________________________________________________________________________
> || Scott A. Selby 6098)o%:::%o(860
> ==== Univ. of Minnesota 098)o%:::%o(8609
> | |__ Dept. of Med/Hematology 6o%:%o(86098)
> | |-.\ Box 480 UMHC (86098)o
> |__| \\ Minneapolis, MN 55455 6098)o%::%o9
> || || 098)o%::::::%o9
> ======__| selby at lenti.med.umn.edu 6o%::::::%o(860
> ________||__ 6o%::%o(8609
>/____________\ o(86098)
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Scott,
Which mdm-2 cDNA do you need? Mouse? There are three human variants?
Did you try the mouse group who first cloned the cDNA:
Fakharzadeh, S.S., Trusko, S.P. & George, D.L. (1991). Tumorigenic
potential associated with enhanced expression of a gene that is
amplified in a mouse tumor cell line. EMBO J., 10(6), 1565-1569.
There are three mdm2 variants in humans:
Sigalas, I. & Lunec, J. Multiple alternate spliced mdm2 transcripts
with loss of p53 binding domain sequences: transforming ability and
frequent detection in human cancer. Unpublished.
Check out GenBank accession U033199. If you need the human probe, just
made some custom oligonucleotides.
