NON-Hayflick fibroblast ageing assay

Edward Krug kruged at ESSEX.HSC.COLORADO.EDU
Fri Oct 28 03:49:31 EST 1994

Hans Selye and others, paraphrased, suggested that ageing is the decreased
range of response to stress.   

I have an approach for producing a physiologically valid Non-Hayflick form of
ageing in fibroblasts in tissue culture.  I have standard tissue culture
facilities and both high and low passage human fibroblasts in culture.  

I am looking for stimulus-response pairs with which to challenge my
fibroblasts and see if they do display a decreased response in absence of
increased passage number.

Any suggestions??

Please send suggestions to me at kruged at essex.hsc.colorado.edu

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