IUBio

(none)

shah at RESUNIX.RI.SICKKIDS.ON.CA shah at RESUNIX.RI.SICKKIDS.ON.CA
Thu Jun 2 12:27:08 EST 1994


I have a technical question:
Are there any assays that can be done on tissue thin sections for
distinguishing between apoptosis and necrosis?
I know there is a method available that probes for generation of DNA
3'-ends by using terminal transferase.  Are there other methods available. 
For example antibodie to proteins that are preferentially induced during
apoptosis.

I also have a question about quiescent (G0 phase) cells.  Are there any 
general markers that could be used with all cell types for in situ
hybridization?

Thank you for cosidering my questions
Sherry

shah at resunix.ri.sickkids.on.ca





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