Dear AGEING LIST subscribers,
I would like to know the current situation for the discovery of the
authors mentioned below, that simple change of composition of the cell
culture medium could eliminate cellular senescence:
Loo, D.T., Fuquay, J.I., Rawson, C.L. and Barnes, D.W. (1987).
Extended culture of mouse embryo cells without senescence: inhibition by
serum. Science 236, 200-202.
These authors demonstrated that normal diploid mouse embryo cells, which
under standard conditions manifest a growth crisis after 7-10 population
doublings, may be successfully cultivated without any sign of an
approaching growth crisis for at least 200 population doublings. All that is
necessary is to change the composition of the culture medium (excluding
blood serum and adding a number of ingredients, including the epidermal
growth factor). In this case the cells, which are apparently capable of
unlimited multiplication, remain diploid and nontumorigenic.
If that is true, then the claim of Dr.Hayflick that all diploid cells
have intrinsic limit to cell division, is definitely wrong.
Does anybody know whether the results of these authors were reproduced
or disproved ?
Does anybody knows the present addresses (E-mail in particular) of these
authors ?
Thanks,
Dr.Leonid A.Gavrilov, Ph.D.
--
________________________________________________________________
-- Dr. Leonid A. Gavrilov, Ph.D. Phone: 7 (095) 427 0047
Principal Research Scientist FAX: 7 (095) 939 0338 or
A.N.Belozersky Institute 7 (095) 939 3181
Moscow State University E-mail addresses:
Moscow 119899 gavrilov at aeiveos.uucp.free.msk.su
Russia libro at genebee.msu.suaeiveos at glas.apc.org
Please send your answer to my most reliable E-mail address:
aeiveos at glas.apc.org
if you wish to be sure to reach me. Thank you !
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